Characterization of Glycopeptides from Physarum Polycephalum Labeled with [3h]mannose or [3h]glucosamine
نویسندگان
چکیده
Glycopeptides from the microplasmodia of Physarum polycephalum grown in the presence of [3H]mannose or [3H]glucosamine were characterized by gel-filtration, anion-exchange and lectin-affinity chromatography before and after enzymatic or chemical treatment. Various types of glycopeptide were found in the growing plasmodia as shown by Con A affinity column chromatography, while pulse-labeled precursors were composed mainly of mannose-rich glycopeptides which were sensitive to endo H and a-mannosidase. During the processing of the glycoprotein, the mannose-rich glycopeptides changed to larger glycopeptides which were resistant to endo H and a-mannosidase. These processing reactions of the glycoprotein appeared to be blocked when the plasmodia underwent differentiation to macrocysts.
منابع مشابه
Effect of thyrotropin-releasing hormone on the carbohydrate structure of secreted mouse thyrotropin. Analysis by lectin affinity chromatography.
Thyrotropin (TSH) is a glycoprotein hormone whose secretion from the anterior pituitary is regulated, in part, by the hypothalamic tripeptide thyrotropin-releasing hormone (TRH). We have used serial lectin affinity analysis to explore whether TRH, in addition to promoting TSH secretion, alters the carbohydrate structure of secreted TSH. Hypothyroid mouse hemipituitaries were incubated in medium...
متن کاملGLYCOPEPTIDES OF HUMAN TERATOMA STEM CELLS Mai
Glycopeptides of human teratoma cells were studied. Two glycopeptide fractions (Fractions A and C) obtained from pronase digests of the human teratoma stem cells of line PA 1 were analyzed. Fraction A glycopeptides were of large size (Mr>7400)• They could be labeled by supplying the cells with 3H—mannose, 3H—fucose, 3H-galactose, 3H—glucosamine, 3H—glucose and 35S—sulfate. Acid hydrolysates of ...
متن کاملCell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas.
The cell surface glycopeptides from an epithelial cell line (CCL 239) derived from normal human colon were compared with those from three cell lines (HCT-8R, HCT-15, and CaCo-2) derived independently from human colonic adenocarcinomas. Cells were incubated with D-[2-3H]mannose or L-[5,6-3H]fucose for 24 h and treated with trypsin to release cell surface components which were then digested exhau...
متن کاملCell Surface Glycopeptides from Human Intestinal Epithelial Cell Lines Derived from Normal Colon and Colon Adenocarcinomas1
The cell surface glycopeptides from an epithelial cell line (CCL 239) derived from normal human colon were compared with those from three cell lines (HCT-8R, HCT-15, and CaCo-2) derived independently from human colonie adenocarcinomas. Cells were incubated with o-[2-3H]mannose or L-[5,6-3H]fucose for 24 h and treated with trypsin to release cell surface compo nents which were then digested exha...
متن کاملVitamin A modifies the glycopeptide composition of cultured Sertoli cells.
Sertoli cells obtained from prepubertal rat testes were cultured in the presence or absence of retinol. Incorporation of monosaccharides and glycopeptide composition of the cells were studied under two experimental conditions. The results indicate that retinol increases the amount of mannose and glucosamine incorporated into cellular glycoconjugates. The labeled glycopeptides obtained from cont...
متن کامل